Observation of a Variant Creatine Kinase Isoenzyme in Sera and Breast Tumor Cytosols
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چکیده
منابع مشابه
Detection of cardiac-specific creatine kinase isoenzyme in sera with normal or slightly increased total creatine kinase activity.
We describe a spectrophotometric kinetic assay for detecting creatine kinase MB isoenzyme activity in the 1 to 10 U/liter range. The MB isoenzyme was isolated [Clin. Chem. 20, 36 (1974)] and assayed (Rosalki method) with an Abbott ABA-100. Good reproducibility was demonstrated for MB isoenzyme activities near 1 U/liter (CV = 2.6%). Sera with normal or slightly increased total creatine kinase ac...
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A radioimmunoassay was developed to measure serum levels of the B isoenzyme of creatine kinase(ATP: creatine N-phosphotransferase, EC 2.7.3.2) (CPK) in order to evaluate the time course and frequency of MB isoenzyme elevation in patients with acute myocardial infarction. The method can identify as little as 0.2 ng of the B portion of the CPK-MB isoenzyme, does not significantly crossreact with ...
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Sir, Creatine Kinase (CK) E.C. 2.7.3.2. is separable into three different isoenzymes by a number of methods. Each isoenzyme is a dimer composed of two subunits (Bor M) each having a relative molecular mass of 40,000. These isoenzymes are widely distributed in tissues, CK-MM and CK-MB are found mainly in the skeletal and heart muscles and CK-BB in G.l. tract and the brain with small amounts also...
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The routine determination of creatine kinase (CK; EC 2.7.3.2) isoenzymes has recently gained acceptance as a diagnostic tool in laboratory medicine (13). Electrophoreticaily three distinct CK isoenzymes can be separated (4, 5). These dimeric bands have been identified throughout the literature as MM, MB, and BB, and originate mainly from skeletal muscle, heart, and brain tissues, respectively. ...
متن کاملCreatine kinase isoenzyme--antibody reactions in immuno-inhibition and immunonephelometry.
I have raised, in rabbits, antibodies against MM and BB isoenzymes of creatine kinase. The antibodies produced were homogeneous by Ouchterlony double immunodiffusion and did not cross react with their opposite antigens. Both antisera, however, appeared to be mixtures of antibodies, displaying different equivalences for activation, inactivation, and, possibly, precipitation. Inactivation studies...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 1980
ISSN: 0002-9173,1943-7722
DOI: 10.1093/ajcp/74.3.332